CHARACTERIZATION AND PHYSICAL STABILITY EVALUATION OF SNAKEHEAD FISH (OPHIOCEPHALUS STRIATUS) POWDER NANOEMULSION

Snakehead fish powder containing albumin has macromolecule size which is difficult to penetrate into membrane cell. So, It was formulated into nanoemulsion utilizing the best comparison of surfactant, co-surfactant and oil. Nanoemulsion of snakehead fish powder was characterized by particle size analyzer. The snakehead fish were extracted by atomizer (-40 °C, 1atm) to gain powder which was formulated into nanoemulsion by spontaneous emulsification method using the best comparison of oleic acid, propylene glycol and Tween 80 (1:10). Propylene glycol was added into Tween 80 and oleic acid and stirred until homogeneous mixture. Then water, containing 0.125% of snakehead fish powder, was added drop by drop until becoming a transparent solution by sonication. It was characterized by UV-Vis Spectrophotometry and Particle Size Analyzer. The results of this research showed that snakehead fish nanoemulsion generated clear, stable, and translucent formula having the transmittance value of 100%. The characterization results described nanoemulsion had the average particle size of 147.63 nm. Meanwhile, the average of polydispersity index and zeta potential values were 0.234 and 13.38 mV. This means that nanoemulsion was stable having a uniform particle size, pH 5.5, and the viscosity value of 136.7 cP. The results of the evaluation and preparation stability test showed a good level of stability with the viscosity and pH by one way ANOVA analysis which did not change significantly.