CHARACTERIZATION OF ENDOGLIN GENE USING IN SILICO TOOLSAbstract
Diabetic retinopathy (DR) is a micro vascular disorder affecting the retina of the eye. Many genes have been identified for its pathogenesis. Endoglin (ENG) is one of the candidate gene involved in DR. Several literature studies helped us understand that the in vitro and in vivo studies of these candidate genes had demonstrated the role of a new gene called endoglin (ENG) capable of inhibiting angiogenesis. In this present study we characterized the physicochemical and functional properties of endoglin gene using bioinformatics tools as this had not been studied previously. The three dimensional structure of endoglin was also computed using homology modelling and the reliability of the model was assessed by predicting its secondary structure along with validation. Additionally, using experimental evidence score, phylogenetic analysis and protein-protein network was framed between endoglin and its interacting neighbouring partners. These results are innovative and relevant enough to start prospective studies that will allow us to establish the relative strength of the prediction of diabetic retinopathy according to the endoglin levels presented by the patient.
V.G. Vidhya * and A. Bhaskar
Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu, India.
07 February, 2017
12 April, 2017
26 April, 2017
01 September, 2017