CHEMICAL COMPOSITION, ANTIOXIDANT, ANTIBACTERIAL ACTIVITY OF ISOLATED OIL AND METHANOL EXTRACT OF TRIDAX PROCUMBENS L.

Tridax procumbens L. is an annual herb that has been used in Ayurveda, Unani, and traditional medicine in India to cure various microbial infections, skin diseases, bronchial disorders, and wound healing. The current study aims to investigate the chemical composition, antioxidant, and antibacterial activities of isolated oil (denoted as TPMG oil) from methanol extract using column chromatography. GC-MS analysis identified a total of six compounds among which 2-pyrrolidinone, 1-methyl (48.68 %), 2, (4H)- benzofuranozone, 5, 6,7, 7a-tetrahydro-4, 4-dimethyl (31.94 %), 2, 2-Dimethoxybutane (8.46%). The antioxidant capacity of TPMG oil with the IC₅₀ value 18.34 μg/mL and significance at P < 0.05 was investigated. The oil was found to be potent bactericidal activity at MIC of 125 µg/mL and 250 µg/mL for S. aureus and P. aeruginosa respectively. Subsequently, the MIC of methanol extract was varied between 125-62.5 µg/mL against selected microorganisms. The ZOI 16.2 ± 0.8366 mm and 15.8 ± 0.8360 mm against P. mirabilis and A.niger were observed. It could be used as an alternative source of antimicrobial and antioxidant agents with the potential drug in the preparation of herbal, and pharmaceutical industrial applications.