CLONAL MICROPROPAGATION OF THREATENED MORINGA CONCANENSIS NIMMO (MORINGACEAE): SUSTAINABLE ALTERNATIVEAbstract
Moringa is one of the medicinally important genera and Moringa concanensis Nimmo is a threatened species of it. Clonal micropropagation has been used to produce true to type multiple copies and help to conserve a number of plants. A comprehensive micro-propagation protocol has been developed in the present study. It was found that Murashige and Skoog (1962) (MS) basal elements enriched with 0.05 mg/L of 1-naphthalene acetic acid (NAA) in combination with 0.3 mg/L of 6-benzyl amino purine (BAP) induced 93.3% shooting and maximum (5.5 ± 1.63) number of micro-shoots. Shoot elongation and multiplication were recorded to enhance by making use of 1.0 mg/L of Gibberellic acid (GA3). Maximum rooting (83.3%) was observed on half-strength MS basal media supplemented with 0.5 mg/L of Indole 3-Butyric Acid (IBA) in combination with 0.5 mg/L NAA. Even though the success of this protocol is varied according to genotype, it still has high applicability for the conservation of germplasm of plant.
Y. Master * and V. Somani
Department of Bioscience, Veer Narmad South Gujarat University, Surat, Gujarat, India.
12 July 2019
22 November 2019
29 February 2020
01 June 2020