COMPARATIVE ANALYSIS OF HPTLC, SECONDARY METABOLITES AND ANTIOXIDANT ACTIVITIES OF TINOSPORA CORDIFOLIA STEM POWDERS

Aim of this study was to develop a HPTLC fingerprint profiles of Tinospora cordifolia stems powdered by different procedures viz; Sample 1 (fresh aqueous powder), 2 (freeze drying powder), 3 (aqueous freeze drying powder) and Sample 4 (dry powder). The study also includes qualitative, quantitative secondary metabolites and the antioxidant potential of all the samples. Result of various physiochemical and phytochemical screening shows a variation among all powdered stems. HPTLC profile revealed a similarity between the samples 1, 2 and 3 while sample 4 shows much lesser no. of constituents. TLC profile of all the samples followed a similar trend like HPTLC fingerprinting. Besides this, secondary metabolite profiles viz; total phenolics, flavonoids, proanthocyanidin and anthocyanidin content of these samples reveals that maximum phenolics and flavanoids content in sample 1 (51.5 µg GAE/mg Wt) and 3 (14.0 µg QE/mg Wt). However, sample 4 was greatly enriched with maximum proanthocyanidin (27.7 µg CE/mg Wt) and anthocyanidin (175 µg CE/mg Wt) contents. Antioxidant potential of these powders was evaluated by total antioxidant, reducing power and DPPH assay which was maximum in sample 4 (170.8, 27.7 µg ascorbic acid/ mg Wt and 61.08 % respectively). A positive correlation between the sum of polyphenolic content and total antioxidant activity were also checked. Sample 4 shows a maximum amount of polyphenols (213.31 µg /mg Wt) and total antioxidants (170.8 µg ascorbic acid/mg Wt) than others. The presence of high levels of polyphenolic contents could be a possible reason behind the higher amount of total antioxidants