COMPARATIVE ANTIBACTERIAL STUDY OF DIFFERENT EXTRACT OF CLAVERIA ROSEA FRIES AGAINST GRAM NEGATIVE AND GRAM POSITIVE PATHOGENS

Objective: To compare the antibacterial activity of different solvent extract of Clavaria rosea.

Method: Different solvent extract of Claveria rosea was prepared. Standard cultures of Escherichia coli (MTCC-1698), Klebsiella pneumoniae (MTCC-7028), Pseudomonas aeuroginosa (MTCC-1934), Salmonella typhi (MTCC-733), Xanthomonas campestris (MTCC-2286), Pseudomonas syringae (MTCC-1604) and Agrobacterium tumefaciens (MTCC-431), gram negative.  Staphylococcus aureus (MTCC-902) and Streptomyces pneumoniae (MTCC-4734), gram positive were used for the study. The antibacterial tests used were the agar well plate method. Tetracycline and Ciprofloxacin was used as the positive control.

Results: The chloroform and methanol extract of Claveria rosea does not show any activity against X. campestris, E. coli, S. typhi, P. aeuroginosa and P. syringae at all concentration in gram negative pathogens. Whereas petroleum ether extract cannot show against S. pneumoniae at all concentration in gram positive pathogens. However, the activity was less than the standard Tetracycline and Ciprofloxacin. The extract shows increasing inhibitory activity with increase in concentration (50%-100%).

 Conclusions: The use of natural products including medicinal mushrooms is increasing day by day and the growth of the medicinal mushroom for this reason our investigation, for screening different solvent extract of Claveria rosea the results obtained confirmed therapeutic potency of some mushroom used in traditional medicine. The mushroom could be potential source of new antimicrobial agent.