COMPARATIVE STUDY OF ANTIOXIDANT ACTIVITY BETWEEN ETHANOLIC AND AQUEOUS EXTRACT OF CLEOME RUTIDOSPERMAAbstract
Cleome rutidosperma is traditionally used in the treatment of paralysis, epilepsy, convulsions, spasm, pain and skin disease. Extraction of the aerial parts of the plant Cleome rutidosperma using water and ethanol and evaluation of their antioxidant activity has been envisaged in this present research work. Both the extracts exhibits significant antioxidant activity in DPPH, Nitric oxide and hydroxyl radical induced In-vitro assay methods. The DPPH radical inhibition (%) was 53.13, 57.13 and 79.12 for CRA, CRE and ascorbic acid respectively. The Cleome rutidosperma extracts (CRA and CRE) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250μg/ml, showing 29.22% and 63.32% of NO inhibition, respectively. The CRA and CRE extracts (25-400μg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/H2O2 system, when compared to that of ascorbic acid.
Anup K. Chakraborty*, Manoj S. Charde, Harekrishna Roy, Satyabrata Bhanja and M. Behera
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jeypore College of Pharmacy, Rondapalli, Jeypore, Koraput, Odisha, India
29 May, 2010
21 September, 2010
03 October, 2010
01 November, 2010