COMPARATIVE STUDY OF IMMUNOMODULATORY ACTIVITY OF OCIMUM SPECIES IN MICEAbstract
Aim: To compare the immunomodulatory effect of Ocimum sanctum and Ocimum basilicum in mice.
Methods: Acute oral toxicity test was performed as per OECD -23 guidelines. Maximum dose level of 2000 mg/kg was selected for toxicological evaluation. Delayed type hypersensitivity reaction were carried out for assessment of cell mediated immune functions
Result: Administration of Ocimum sanctum and Ocimum basilicum did not produced any mortality or any signs of behavioral changes or toxicity at the dose level of 2000 mg/kg body weight in mice. The result of the present study showed that acute administration of extract of Ocimum sanctum and Ocimum basilicum may be safe as the LD50 may be greater than 2000mg/kg. Treatment with Ocimum sanctum enhanced DTH reaction, which is reflected from foot pad thickness when compared with Ocimum basilicum and control groups whereas a similar effect was observed when compared to the reference drug Aswagandha choorna, which is a well-known immune booster.
Conclusion: Increase in DTH reaction in mice in response in T cell dependent antigen revealed the stimulatory effect of Ocimum sanctum on T cells.
R. Caroline Jeba* and G. Rameshkumar
Department of Industrial Biotechnology, Dr. M. G. R Educational and Research Institute University, Maduravoyal, Chennai-600 095, Tamil Nadu, India
02 May, 2013
07 June, 2013
21 August, 2013
01 September, 2013