COMPARISON OF HPLC AND HPTLC TECHNIQUES FOR DETERMINATION OF UMBELLIFERONE FROM DRIED TUBER POWDER OF IPOMOEA MAURITIANA JACQ.Abstract
Umbelliferone is a widely occurring phenolic compound of plant origin, for which many biological activities against chronic diseases have been reported. A comparative study of HPLC and HPTLC method has been developed for the quantitation of umbelliferone from dry tuber powder of Ipomoea mauritiana Jacq. Both the methods were validated for linearity, precision, limit of detection (LOD), limit of quantitation (LOQ), system suitability and accuracy in accordance with ICH guidelines. The accuracy of the HPLC and HPTLC method was checked by a recovery study conducted at three different levels and values of the average percentage recoveries for these two methods were found to be 97.90 and 98.90 respectively for umbelliferone. The study showed that both the methods are of similar efficiency, sensitivity and can be used for determination umbelliferone from dried tuber powder of Ipomoea mauritiana Jacq.
Vidya Dighe and Shreeda Adhyapak*
Department of Chemistry, Ramnarain Ruia College, Matunga, Mumbai- 400080, Maharashtra, India
10 July, 2011
21 August, 2011
25 October, 2011
01 November, 2011