COMPLEXES OF COBALT(II), NICKEL(II) AND ZINC(II) WITH SCHIFF BASES DERIVED FROM 4-ANISALDEHYDEAbstract
A new spectrophotometric method is developed for the determination of artesunate (ARTS) in pharmaceutical preparation and bulk drug. The method involved heat-catalyzed derivatization of the drug with acetic anhydride and concentrated sulphuric acid producing a yellow coloured product peaking at 420.6 nm. The optimum conditions of the reaction and the analytical procedures of the assay were established. Under the optimum conditions, linear relationship with good correlation coefficient (0.9994) was found between the absorbance and the concentration of ARTS in the range of 4 – 60 µg mL-1. The limit of detection was found to be 0.72 µg mL-1 and quantitation limit was 1.8 µg mL-1. The analytical performance of the method was validated and the results were satisfactory. The developed method was successfully validated and applied for the determination of artesunate in bulk and pharmaceutical formulation without any interference from common excipients.
S. Meena and S. M. Sandhya*
Department of Pharmaceutical Analysis, Devaki Amma Memorial College of Pharmacy, Pulliparamba P.O, Malappuram – 673634, Kerala, India
13 August, 2012
27 September, 2012
29 November, 2012
01 December, 2012