CYCLOPHOSPHAMIDE INDUCED DNA AND TESTICULAR DAMAGE: PROTECTIVE ROLE OF WITHANIA SOMNIFERAAbstract
Objectives: Cyclophosphamide is an alkylating agent known for its reproductive toxicity in males. This study evaluates the protective effects of Withania somnifera extract (WSE) on cyclophosphamide (CP) induced testicular toxicity and DNA damage in male rats. Methods: Adult male rats were randomly divided into four groups Group1-Control, Group 2- Cyclophosphamide, Group 3- Withania somnifera extract, Group4- Cyclophosphamide+ Withania somnifera. After the experimental period of 8 weeks, sperm quality, hormonal analysis, and DNA damage studies were carried out. Histopathological analysis of testes was performed for the assessment of spermatogenic disorders. Results: In CP-treated rats, sperm quality was reduced with decreased testosterone levels with increased DNA damage. Co-administration of WSE with CP improved sperm quality, testosterone levels, spermatogenesis and genotoxicity of cyclophosphamide. The histopathological study also restored most of the degenerative and necrotic changes. Conclusions: This study suggests a prominent role of WS against CP-induced testicular toxicity and DNA damage.
Y. Sunil Kumar, N. Suchetha Kumari, K. P. Sharmila and Divya Bhargavan *
Department of Pharmacology, K. S. Hegde Medical Academy, Derlakatte, Mangalore, Karnataka, India.
27 July 2022
27 August 2022
20 October 2022
01 April 2023