CYTOPROTECTIVE ROLE OF ENCAPSULATED ASTAXANTHIN AGAINST HEPATO-CELLULAR CARCINOMA (HepG2) CELL LINEAbstract
Astaxanthin (3,3′-dihydroxy-β, β′-carotene-4,4′-dione) is a xanthophyll carotenoid, which is found in Haematococcus pluvialis, Chlorella zofingiensis, Chlorococcum, and Phaffiarhodozyma. Astaxanthin play major role as a nutritional supplement, antioxidant and anticancer agent, anti-diabetes, cardiovascular diseases, and neurodegenerative disorders. In our previous study, astaxanthin was first encapsulated by different agents and characterized using SEM, FT-IR, and in-vitro drug release. The present study aimed to explore the anticancer activity of encapsulated and non-encapsulated astaxanthin on the African green monkey normal kidney Vero cell line and HepG2 cell line. From the in-vitro study, the IC50 of HepG2 cells was found to be 500 μg/ml compared to the normal Vero cell line with IC50 of 600 μg/ml of which was confirmed by MTT assay. Further, the fluorescence microscope and DNA fragmentation were carried to analyze the apoptosis in HepG2 treated cells. Thus, our results suggested that liposomal encapsulated astaxanthin (ME1) exhibits good anticancer activity when compared amongst other test samples and positive drug sorafenib.
V. Suganya and V. Anuradha *
PG and Research Department of Biochemistry, Mohamed Sathak College of Arts & Science, Sholinganallur, Chennai, Tamil Nadu, India.
05 January 2020
08 April 2020
11 April 2020
01 January 2021