CYTOTOXIC ACTIVITY OF N-HEXANE, CHLOROFORM AND CARBON TETRACHLORIDE FRACTIONS OF THE ETHANOLIC EXTRACT OF LEAVES AND STEMS OF BACCAUREA RAMIFLORA (LOUR.).Abstract
The purpose of the study was to find out the cytotoxic activity of the n-hexane, chloroform and carbon tetrachloride fractions of the ethanolic extracts of the leaves and stems of Baccaurea ramiflora (Lour.). Ethanolic extracts of the leaves and stems of Baccaurea ramiflora were subjected to solvent-solvent partitioning using n-hexane, chloroform and carbon tetrachloride to obtain n-hexane leaves fraction, chloroform leaves fraction, n-hexane stems fraction, chloroform stems fraction and carbon tetrachloride stems fraction. Each fraction was assayed for their cytotoxic effect using brine shrimp lethality bioassay. Among the fractions, the n-hexane fractions of leaves and stems showed significant cytotoxic effects having LC50 values of 7.79 µg/ml (95% confidence interval 6.48-9.37) and 5.78 µg/ml (95% confidence interval 4.76-6.99) respectively as compared to vincristine sulfate (LC50= 2.81 µg/ml (95% confidence interval 1.97-4.01) which was used as positive control. The results support the traditional uses of B. ramiflora for various medicinal purposes and thus demand the isolation and identification of active principles and thorough bioassay.
Md. Amran Howlader , Apurba Sarker Apu*, Repon Kumer Saha , Farhana Rizwan , Nishat Nasrin , Muhammad Asaduzzaman
Department of Pharmacy, East West University, 43, Mohakhali C/A, Dhaka-1212, Bangladesh
28 October, 2011
15 February, 2012
26 February, 2012