CYTOTOXICITY OF EXTRACTS FROM THE PHILIPPINE MARINE SPONGES: PARATETILLA SP., STYLISSA SP. AND PETROSIA (STRONGYLOPHORA) NIGRICANSAbstract
This paper reports the cytotoxic activities of the hexane, dichloromethane, ethyl acetate, and methanol fractions of the marine sponges: Paratetilla sp., Stylissa sp., and Petrosia (strongylophora) nigricans, collected from the Island waters of Lipata, Surigao del Norte, Philippines, using the enzymatic reduction of a yellow tetrazolium salt, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) against four cancer cell lines HCT116 (colon), MCF7 (breast), HepG2 (liver) and A549 (lung). The assay revealed that most of the fractions are favorably active to only HCT116; except for the dichloromethane fraction of Stylissa sp., which demonstrated remarkable cytotoxicity against MCF7 and HCT116 with % viability of 15.72±0.03% and 21.48±0.07%, respectively, after treatment with 30µg/mL of the sponge extract.
H. Luyao * and M. Uy
Department of Chemistry, Mindanao State University-Iligan Institute of Technology, Iligan City, Philippines.
08 October 2020
01 September 2021
29 September 2021
01 October 2021