DESIGN AND VALIDATION OF NOVEL UV-VISIBLE SPECTROSCOPIC METHOD FOR ASSAY OF AMPHOTERICIN B
AbstractThe objective of this research was to design and validate an alternative accurate method for quantitative determination of Amphotericin B. Amphotericin B deoxycholate life-saving drug belongs to the polyene class approved for the treatment of progressive and potentially fatal fungal infections. Amphotericin B was estimated at 382 nm and the percentage recovery was found to be 100.2 ± 0.34. The method was tested and validated for various parameters according to the ICH guidelines. The repeatability (inter-day) and intermediate precision (intra-day) precision studies revealed that the method is precise and reliable where all the RSD values were less than 2% for the routine analysis of drug. The limit of detection and limit of quantitation were 0.5μg/ml and 1.5μg/ml respectively. Results shows that the developed method is simple, reproducible and successfully for the estimation of Amphotericin B without the interference of common excipients.
Article Information
16
2734-2739
787 KB
132
English
IJPSR
Rajiv Kumar *, Rajan Salwan, Satwinder Kaur, Ankit Tangri and Muskan Sharma
Khalsa College of Pharmacy, Amritsar, Punjab, India.
rajiv042@rediffmail.com
26 March 2024
02 May 2024
18 May 2024
10.13040/IJPSR.0975-8232.15(9).2734-39
01 September 2024
Download