DETECTION OF B. ABORTUS S19 ON UTERINE RAT (RATTUS NORVEGICUS) WITH IMMUNOHISTOCHEMICAL METHODAbstract
Objective: Brucellosis in humans is the most common zoonotic infection in the world. Brucellosis can be prevented by vaccination but no effective vaccines are known for prevention of human brucellosis and minimal impact of animal brucellosis. Outer membrane proteins (OMPs) of Brucella sp. have been the focus of vaccine development and the diagnosis of brucellosis. Methods: The pathogenesis study of brucellosis used rat by intraperitoneal injection. Profile OMP detected by SDS-PAGE. The antibodies took from rabbit and the immune response did with western blotting, dot blot and immuno histochemistry. Results: B. abortus S19 cell to the rat show the presence of inflammatory cells and bleeding in the uterine rat. The 36 kDa OMP could isolated from B. abortus S19. Rabbit antibody to 36 kDa OMP can detect B. abortus of this histological of the uterus. OMP 36 kDa B. abortus S19 is antigenic characterized by its ability to stimulate antibodies in the host’s body (Oryctolagus cuniculus). Conclusion: B. abortus S19 can be detected in histopathology of rats uterus shown by interaction of antigen and antibody by using immunohistochemical method.
F. Purnamawati *, S. Santoso and Aulanni’am
Biomedical Science Master Program, Medical Faculty, Universitas of Brawijaya Malang, Indonesia.
24 December, 2017
15 February, 2018
04 March, 2018
01 September, 2018