DETECTION OF ETHYLENE FOR IN VIRO EVALUATION OF THE UV PHOTOPROTECTIONAbstract
In this study, the antioxidant capacity during UV exposure has been investigated by using a laser-based trace gas detector for the detection of gaseous ethylene as an indicator for lipid peroxidation. Biocream was used as a control and ascorbic acid, butylated hydroxytoluene, phloroglucinol and hydroquinone were added as antioxidants in a mixing concentration of 2.5% (w/w). The experimental results show that there is a decrease in the number of ROS due to the presence of antioxidants, from which ascorbic acid is the most effective. The detection method offers a direct and on-line way to evaluate the capability of antioxidant in reducing the harmful effect of the UV radiation.
I. E. Santosa , A.N.I. Harnita , S. Martono , Sudarsono , S. Widyarini and F.J.M. Harren*
Life Science Trace Gas Facility, Molecular and Laser Physics, Radboud University, Nijmegen, the Netherlands
11 November, 2012
23 December, 2012
15 February, 2013
01 March, 2013