DETERMINATION OF PRILOCAINE HCl IN PHARMACEUTICAL PREPARATION AND HUMAN PLASMA WITH SPECTROPHOTOMETERYAbstract
A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230 nm. The method was linear in concentration range of 3-15 μg/mL for standard solution and 4-15 μg/mL for human plasma. Linearity was determined by calculating correlation coefficient. These values were found as a 0.9999 and 0.9967 in standard solution and human plasma, respectively. Developed spectrophotometric method was found suitable in terms of accuracy, sensitivity, precision, reproducibility. In addition to these, this method could be easily and directly applied to both human plasma and pharmaceutical preparation.
Alptug Atila* and Yucel Kadioglu
Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey
13 December, 2011
12 January, 2012
29 March, 2012