DETERMINATION OF RESIDUES OF FLUOROQUINOLONES IN POULTRY MEAT BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYAbstract
Three analytical methods were developed for the determination of selected fluoroquinolones in chicken meat by high performance liquid chromatographic technique. In each method, we took two fluoroquinolones (Danofloxacin and difloxacin; Ciprofloxacin and enrofloxacin; Ofloxacin and pefloxacin) research study. Rapid, simple and sensitive sample treatment procedures were developed and used to extract the fluoroquinolones from the chicken tissues with good recoveries. Extraction recovery from chicken tissues was in the range of 86.96 to 97.49 %, depending upon the analytes. The shape of the curves for all drugs was linear in the investigation concentration range 10-1000 ng/ml, and the correlation coefficient of the linearity curves for all drugs was in the range of 0.9707- 0.9991. The detection limits for fluoroquinolones in chicken tissues were well below the Maximum Residual Limits (MRLs) for antibiotics. The limit of detection (LOD) was in the range of 9-12 µg/kg, and the limit of quantification (LOQ) was in the range of 30-40 µg/kg for all analytes in chicken tissues. The separation results and parameters of the methods were evaluated from spiked tissue samples, and results were found good and within range. The proposed methods can be applied for routine determination of these fluoroquinolones in chicken tissue samples.
Rajeev Sharma * and Reena S Lawrence
Department of Chemistry, Sam Higginbottom University of Agriculture, Technology & Sciences, Prayagraj Allahabad, Uttar Pradesh, India.
18 January 2021
19 July 2021
20 July 2021
01 December 2021