DETERMINATION OF TOTAL FLAVONOID AND PHENOL CONTENT IN MIMUSOPS ELENGI LINN.Abstract
Objective: To study qualitative and quantitative estimation of phytochemicals and determination of total phenol and flavonoid content in plant parts of Mimusops elengi Linn. Methods: The solvents methanol, ethanol, aqueous and petroleum ether are used for extraction of Mimusops elengi Linn. plant parts. Phytochemical screening of plant parts was carried out in all the solvents. Quantitative estimation of carbohydrate, protein and lipids was done according to the established protocol. Determination of total phenol content was carried out using Folin – Ciocalteau method and total flavonoid content using Aluminium chloride colorimetric method. Results: The result revealed the presence of carbohydrates, alkaloids, flavonoids, tannin, saponin and terpenoids etc. Total phenol content was expressed in mg of Gallic Acid Equivalent (GAE) per g of dry weight. In results it was found that petroleum ether extract shows highest phenol content 267.20 mg/g in fruits. The content of flavonoids was expressed in mg of Quercetin Equivalent (QE) per g of dry weight. It was evaluated that total flavonoid content found highest in bark 113.35 mg/g in petroleum ether extract. Conclusion: These results suggest that Mimusops elengi Linn. plant is medicinally and commercially important.
R. Mathur and R. Vijayvergia*
Plant Pathology and Biochemistry Laboratory, Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India.
03 April, 2017
11 August, 2017
17 September, 2017
01 December, 2017