DEVELOPMENT AND CHARACTERIZATION OF SOLID LIPID NANOPARTICLES BY SOLVENT DIFFUSION- EVAPORATION METHOD FOR TOPICAL DELIVERYAbstract
The aim of the present study was to prepare solid lipid nanoparticles (SLNs) for the topical delivery. Tristearin was used as solid lipid with soya lecithin by using surfactant, Poloxamer 188 (1%) and Tween 80 (0.5%). Solid lipid nanoparticles (SLN) loaded with Flucanazole were prepared by solvent diffusion- emulsification method. The properties of the SLNs such as particle size, zeta potential (ZP), Polydispersity index (PI) and drug % entrapment efficiency (% EE) were investigated. The morphology of SLNs was observed by transmission electron microscopy (TEM) and Scanning electron microscopy (SEM). The drug release behavior was studied by in vitro method using franz diffusion cell with dialysis membrane. The results show the formulation F2 had smallest particle size of 122±3.42 nm with Zeta potential -24.03±1.84 and Polydispersity index 0.668±3.21. The % Entrapment efficiency of formulation F2 was found to be 76.53±0.24. The average particles sizes of the nanoparticles were found to increase on storage, which may be due to aggregation of particles. This effect was encountered lower in the case of formulation stored at 4oC, which signify that aggregation can be regulated by regulating temperature and hence ideal storage condition of SLNs are at 4oC than those stored at 27oC. Fluconazole-SLNs in vitro drug release was conducted in phosphate-buffered saline (pH 7.4) at 37oC. In vitro cumulative % drug release from F2 SLN formulation was found 56 % in PBS (pH-7.4) over 48 h.
Poonam Yadav*, Girish Soni, Raghuveer Irchhaiya, Alok Mahor and Shashi Alok
Institute of Pharmacy, Bundelkhand University, Jhansi- 284 128, Uttar Pradesh, India
24 September, 2013
10 December, 2013
17 February, 2014