DEVELOPMENT AND IN-VITRO EVALUATION OF CENTELLA ASIATICA AND SILVER SOLUTION COATED SUTURES FOR ANTIBACTERIAL ACTIVITYAbstract
The research aim is to develop and evaluate Centella asiatica Chloroform extract and silver-coated suture against the most common organisms that cause surgical site infections (SSI) by in-vitro methods. This prospective study is to coat the Centella asiatica (CA) extract to the suture along with the silver nitrate solution. In this study, a suture filament was formulated with Poly Lactic Glycolic Acid (PLGA) (50:50 ratio). A formulated filament was coated with Centella asiatica extract, and its property was compared with the marketed future. After minor modifications, the marketed suture was coated with Centella asiatica extract. Antibacterial efficacy of Centella asiatica extract coated suture filaments with and without silver nitrate solution were evaluated against gram-positive and gram-negative bacteria. Chloroform extract of Centella asiatica powder was effective against gram positive and negative bacteria compared to crude powder. Particle size and Zeta potential of the CA powder extract with and without silver nitrate at the end of 3 h was found to be 676.9 and 140.1nm and 0.295 1.45mv of Zeta potential, respectively. The results confirm the addition of silver nitrate reduces the particle size and alters the zeta potential Monofilament PLGA suture didn’t show any significance in antibacterial activity against S. aureus compared to CA with PLGA and AgNO3. When comparing the antibacterial activity of chloroform extract of CA with PLGA and CA Chloroform Extract, the significant activity (P < 0.05) was observed in chloroform extract of CA towards S. aureus compared to E. coli. Prepared PLGA mono filament shows higher antibacterial action compared to multi commercial filament pre-processed marketed l sutures.
Loganathan Thilagavathi, Nithyanth Munusamy and Veintramuthu Sankar *
Department of Pharmaceutics, P. S. G. College of Pharmacy, Coimbatore, Tamil Nadu, India.
26 October 2020
04 October 2021
06 October 2021
01 November 2021