DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR THE ESTIMATION OF HYDROCHLOROTHIAZIDE IN TABLET DOSAGE FORMSAbstract
An accurate, precise and reproducible high performance liquid chromatographic method was developed for quantitative estimation of hydrochlorothiazide in tablet dosage forms. Chromatographic separation of the drug was achieved on a Kromasil C18 column (150 x 4.6 mm; 5µ) using a mobile phase consisting of phosphate buffer (pH 2.5) and acetonitrile (50:50 v/v) at a flow rate of 0.6 mL/min. The drug in the eluate was monitored at 254 nm. The retention time obtained for the drug was 3.47 min. The calibration curve was linear over the range of 20-60 µg/mL of the drug. The performance of the method was validated according to ICH guidelines. The method could be applied for determination of hydrochlorothiazide in its tablet dosage forms without any interference from excipients or endogenous substances. The proposed method is suitable for routine quality control analysis of hydrochlorothiazide.
V. Vijayasree , C. Pallavan and J.V.L.N. Seshagiri Rao*
Yalamarty College of Pharmacy, Tarluwada, Visakhapatnam- 530052, India
19 November, 2012
21 February, 2013
27 February, 2013
01 March, 2013