DEVELOPMENT AND VALIDATION OF AN UV- SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF FLUOXETINE IN PURE AND TABLET DOSAGE FORMSAbstract
A rapid, precise, accurate, sensitive, simple, fast and reliable spectrophotometric method has been developed for determination of Fluoxetine in bulk and pharmaceutical dosage forms. The solubility of Fluoxetine was also examined in various solvents like distilled water, methanol, ethanol, acetonitrile, HCL, chloroform etc. For the determination of working wavelength different concentrations of Fluoxetine (10-60μg/ml) in water: methanol (9:1) was scanned using UV-VIS spectrophotometer within the wave length region of 200-400 nm against water and methanol mixture as blank. For the calibration curve the prepared concentrations were scanned at 216 nm in UV-VIS spectrophotometer. The R2 value was found to be 0.999. Then the standard deviation was found to be 0.0065. Then the stability study was performed against neutral, acidic and basic condition at 60°C as per ICH guideline.
Avisek Mukhopadhyay, Kishanta Kumar Pradhan*, Rojalini Samanta
Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand, India
18 February, 2014
10 April, 2014
13 June, 2014
01 August, 2014