DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR SOME AZOLES IN PHARMACEUTICAL PREPARATIONAbstract
A new, simple and rapid reversed-phase high-performance liquid chromatography (HPLC) method was developed and validated for the determination of some azoles drug (Ketoconazole, Isoconazole and Miconazole) in pharmaceutical dosage forms in Jordan market. The HPLC separation was achieved on a C18 BDS column (100 mm × 4.6 mm) using a mobile phase of acetonitrile and 0.05 M Ammonium Acetate buffer (70:30, v/v) that adjusted to pH 6 using phosphoric acid at a flow rate of 1 mL min−1 and using UV absorbance detection at 254 nm. The method was validated for specificity, linearity, precision, accuracy, robustness. The separation was completed within 11.77 minutes and the assay was linear over the concentration range of 50% to 150% (r2 = 0.9999), the percent recovery for test and reference formulation was (98.14%-101.12%).
W.A. Dayyih*, N. A. saadi, M. Hamad, E. Mallah, K. Matalka and T. Arafat
Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences, Petra University, Amman, Jordan
20 May, 2012
24 July, 2012
20 September, 2012
01 October, 2012