DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF 6-GINGEROL IN HERBAL FORMULATIONS AND EXTRACTSAbstract
A simple, selective, precise and reliable HPTLC method of analysis has been developed and validated for estimation of 6-gingerol in extracts of dried rhizomes of Zingiber officinale and marketed formulations containing Ginger. The method employed TLC aluminium plates precoated with silica gel 60F 254 as stationary phase. The solvent system consisting of n-hexane:ether (40:60v/v). Densitometric analysis was carried out in absorbance mode at 280nm. The method was validated in terms of linearity, precision and accuracy. The LOD and LOQ were 60 and 100ng/spot respectively for 6-gingerol. The average percentage recovery of 6-gingerol was found to be 99.93%. The developed method can be successfully used for identification and quantification of 6-gingerol in extracts and formulations. The method is also suitable for rapid and simple authentication and comparison of differences among samples with identical plant resources but different geographic locations.
K. Senthil Kumar, B. Manasa , Kaliloor Rahman and B. Sudhakar
Sri Adichunchanagiri College of Pharmacy, B.G. Nagara- 571 448, Karnataka, India
17 May, 2012
30 July, 2012
26 September, 2012
01 October, 2012