DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF FLUOXETINE IN ITS CAPSULE FORMULATIONAbstract
An HPTLC method for estimation of Fluoxetine in its capsule formulation has been developed. It employs aluminium backed silica gel 60 F254 TLC plates, (20 cm × 10 cm, layer thickness 0.2 mm) prewashed with methanol and mobile phase comprising of toluene: 2-propanol: ammonia 2:2:0.4 (v/v/v). The developing solvent was run upto 80 mm in Camag chamber previously saturated with 10.0 mL of solvent mixture for 30 min. Densitometric scanning was then performed with Camag TLC scanner-3 equipped with winCATS software Version 1.3.0 at λmax 227 nm. The Rf value was found to be 0.74. The recovery of Fluoxetine was found to be 99.90% ± 1.68. The limit of detection and limit of quantitation were found to be 43.55 ng/spot and 131.99 ng/spot. The % RSD of intra-day variation and inter day variation were 0.54 and 0.41 respectively.
M. S. Bhatia* and S. T. Kumbhar
Professor, Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near chitranagri, Kolhapur, Maharashtra, India
19 March, 2011
11 April, 201
25 May, 2011
01 June, 2011