DEVELOPMENT AND VALIDATION OF NOVEL UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF MEBENDAZOLE IN PHARMACEUTICAL FORMULATIONAbstract
Mebendazole is a broad-spectrum anthelmintic drug that is widely used in the treatment of helminthic infections. A simple, accurate, and precise UV-visible spectrophotometric method has been developed and validated for the determination of Mebendazole in tablet formulation. The solvent used for the development of the proposed method was Phosphate buffer 6.8 at 278.6 nm. The Beers’ law was obeyed in the concentration range 5-30 μg/ml. The developed method was validated as per ICH guidelines such as linearity, accuracy, precision, the limit of detection, the limit of quantification, and robustness. The correlation coefficient was found to be 0.999. The accuracy was found between 99.22 to 99.54%. The % RSD of Mebendazole was found to 0.05 to 0.20 for intraday and 0.05 to 0.15 for inter-day precision. The method was found to be precise as % RSD was found to be less than 2%. The LOD and LOQ were found to be 0.986 µg/ml and 2.988 µg/ml, respectively. The results demonstrated that the excipients in the commercial tablets did not interfere with the method and can be conveniently employed for routine quality control analysis of Mebendazole in pharmaceutical tablets formulations.
S. Shrivastava * and C. D. Kaur
Shri Rawatpura Sarkar Institute of Pharmacy, Kumhari, Durg, Chhattisgarh, India.
03 April 2020
28 August 2020
07 September 2020
01 April 2021