DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF CYTARABINE IN BULK AND PHARMACUTICAL DOSAGE FORMSAbstract
A simple, rapid, accurate, specific and sensitive reverse phase liquid chromatography method (RP-HPLC) was developed and validated for the estimation of Cytarabine in Pharmaceutical dosage form. The method was standardized using a Nova pack C18 column (250 mm x 4.6 mm ID; Particle size 3 μm) and the mobile phase consisted of acetonitrile: buffer (Ammonium acetate) at 30:70% v/v. The eluents were monitored at 272 nm and at 1 ml/min flow rate. The retention time was found to be 2.734 min for Cytarabine. The developed method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantification in accordance with the ICH guidelines. Linearity was obtained with the correlation coefficient value r2 =1 for Cytarabine. The proposed method can be used for the estimation of Cytarabine in bulk and Pharmaceutical dosage forms.
Varanasi. S.N. Murthy*, A. Rohini , K.E. Pravallika , A. Prameela Rani and S.A. Rahaman
Research Scholar, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India
05 July, 2013
13 August, 2013
17 November, 2013
01 December 2013