DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF RITONAVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMSAbstract
A simple, precise, specific and accurate reverse phase HPLC method has been developed for the determination of Ritonavir in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved on Symmetry C18 (4.6 x 100mm, 3.5 μm) column using a mixture of Buffer: Acetonitrile (50:50) as the mobile phase at a flow rate 1.0 ml/min. Linearity was observed in concentration range of 50-150μg/ml. The retention time of Ritonavir was 5.1 min. The analyte was monitored using UV detector at 239 nm. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness.
K. Chiranjeevi* and K. P. Channabasavaraj
Department of Pharmaceutical Analysis, Bharathi College of Pharmacy, Bharathi Nagara, Mandya (District), Karnataka, India
02 November, 2010
21 January, 2011
02 February, 2011
01 March, 2011