DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND INDAPAMIDE IN TABLET DOSAGE FORM
AbstractA simple, specific, accurate and precise reversed phase high pressure liquid chromatographic method has been developed for the simultaneous determination of Amlodipine Besyltate and Indapamide in tablet dosage form by reversed phase C18 column (Phenomenex C18, 5μ, 250 mm x 4.6 mm). The sample was analysed using Methanol: Water in the ratio of 95:5 as a mobile phase at a flow rate of 1.0 ml/min and detection at 238 nm. Calibration curves were linear with correlation coefficient (r2) 0.996 over a concentration range of 2-16 µg/mL for Amlodipine besylate and 0.997 over a concentration range of 1-7 µg/mL for Indapamide. The retention time for Amlodipine besylate and Indapamide was found to be 8.722 and 2.855 min respectively. The mean recoveries were found to be 99.98 ± 1.40 and 100.37 ± 1.25 %. for Amlodipine besylate and Indapamide respectively. The relative standard deviation (RSD) was found to be <2.0 % for both drugs. The proposed method was validated and successfully applied to the estimation of Amlodipine besylate and Indapamide in tablet dosage form.
Article Information
28
3146-3150
519KB
1366
English
IJPSR
Manish C. Raj* and Bharat G. Chaudhari
Department of Quality Assurance, Shree S.K. Patel College of Pharmaceutical Education and Research, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India
manish21288@gmail.com
03 May, 2012
02 June, 2012
27 August, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(9).3146-50
01 September, 2012
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