DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION FOR PIPERINE AND GALLIC ACID IN ZEAL HERBAL GRANULES BY HPTLC METHODAAbstract
A simple HPTLC method has been developed for the estimation of Gallic acid and Piperine in methanolic extract of Zeal herbal granules [ZHG], a Polyherbal formulation. The separation was performed on TLC aluminum plates precoated with silica gel 60 F254, good separation was achieved in the mobile phase of toluene: ethyl acetate: formic acid (11: 15: 1v/v/v) and densitometry determination of Gallic acid (0.50 Rf) and Piperine (0.70 Rf) was carried out at single wavelength scanning at 254 nm. The method validated as per ICH guideline, shows regression co-efficient value for Piperine 0.9992 and 0.9979 for Gallic acid in range of 40-90 µg/mL Recovery of Piperine and Gallic acid in final product found in range of 98-102%. Precision study (interday & intraday) showed that the relative standard deviation is less than 2%, showing method is well precise. Quantification study shows 0.23 % w/w of Piperine and 0.37 % w/w of Gallic acid in ZHG. Present study shows that developed method is simple, precise, specific, robust and accurate, and could find application in routine quality-control analysis of Polyherbal formulation.
A. Shah , N. Gurav*, B. Solanki 2, P. Patel 2 and J. Modi
Vasu Research Center, A Division of Vasu Healthcare Pvt. Ltd., 896/A GIDC, Makarpura, Vadodara, Gujarat, India
10 April, 2013
10 May, 2013
27 July, 2013
01 August, 2013