DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF GLICLAZIDE AND METFORMIN HYDROCHLORIDE IN BULK AND TABLET DOSAGE FORM BY SIMULTANEOUS EQUATION METHODAbstract
A simple, rapid, accurate, precise, specific and economical spectrophotometric method for simultaneous estimation of Gliclazide (GLC) and Metformin hydrochloride (MET) in combined tablet dosage form has been developed. It employs formation and solving of simultaneous equation using two wavelengths 227.0 nm and 237.5 nm. This method obeys Beer’s law in the employed concentration ranges of 5-25 μg/ml and 2.5-12.5 μg/ml for Gliclazide and Metformin hydrochloride, respectively. Results of analysis were validated statistically and by recovery studies.
Ketan P. Dadhania*, Parthika A. Nadpara , Yadvendra K. Agrawal
Smt. R. D. Gardi B. Pharmacy College, Opp. Garden Dinner Club, Rajkot-Jamnagar Highway, Nyara, Rajkot, Gujarat, India
17 March, 2011
21 April, 2011
18 May, 2011
01 June, 2011