DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RITONAVIR IN TABLET DOSAGE FORMAbstract
A simple, precise, accurate and repeatable method for bulk and tablet dosage form of Ritonavir have been developed using spectrophotometric method. Differential spectrophotometric method was used. The developed method was validated according to ICH (Q2R1) guidelines and was found to be accurate, precise and specific. Amplitude difference was taken at absorbance maxima at 246 nm and absorbance minima at 266 nm. Linearity range was found to be within the concentration range of 10-30 μg/mL. Limit of detection and quantification was found to be 2.62 and 7.96 μg/mL respectively. The proposed method was found accurate in the range of 95.5 to 105.5%. It can be successfully applied for the estimation of Ritonavir in bulk and pharmaceutical dosage forms. Results of the analysis were validated statistically and by recovery studies.
C.D. Trivedi*, R. B. Mardia, B.N. Suhagia and S.P. Chauhan
Faculty of Pharmacy Dharamsinh Desai University, Nadiad- 387001, Gujarat, India
05 July, 2013
25 August, 2013
16 November, 2013
01 December 2013