DEVELOPMENT AND VALIDATION OF THE KINETIC-SPECTROPHOTOMETRIC METHOD FOR THE QUANTITATIVE DETERMINATION OF MEZLOCILLIN IN PURE SUBSTANCE AND MEDICAL PREPARATIONAbstract
The kinetic of conjugated reactions of S-oxidation and perhydrolysis of Mezlocillin (Mezl) with potassium caroate in alkaline medium is studied by the increase of forming product light absorbance at 290 nm. Baypen® – powder Mezl sodium monohydrate in flacons for preparation of solution for injections (Mezl 1.0 g). Peroxomonosulfate acid as triple potassium salt 2КНSO5∙КНSO4∙K2SO4 (Oxone®) of “extra pure” qualification was used as oxidant. A scheme of peroxoacid oxidation and perhydrolysis conjugated reactions of Mezl by means of potassium caroate is proposed. A new spectrophotometric method for quantitative determination of sodium Mezl in Baypen ® preparation using potassium caroate (KHSO5) as analytical reagent was proposed. At pH 3-4 for 1 mole of penicillin, 1 mole of KHSO5 is consumed, the quantitative interaction is achieved within a time of more than 1 min (observation time). The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. RSD = 1.32%, δ = +0.49%. It can be seen that Mezl can be determined successively with good accuracy and reproducibility. The new procedure was developed and ability of quantitative determination of penicillin in pharmaceutical preparation Baypen® by spectrophotometric method using potassium caroate (KHSO5) as analytical reagent was shown.
S. P. Karpova *, A. A. Krutskikh, O. O. Mozgova and O. V. Shovkova
Department of Physical and Colloid Chemistry, National University of Pharmacy, Pushkinska, Kharkiv, Ukraine.
28 June 2019
23 November 2019
08 February 2020
01 May 2020