DEVELOPMENT AND VALIDATION OF THE METHOD FOR THE SIMULTANEOUS ESTIMATION OF ACECLOFENAC AND PANTOPRAZOLEAbstract
In the present research work, a new UV spectroscopic and RP-HPLC method was described for simultaneous estimation of Aceclofenac (ACL) and Pantoprazole (PNT). Simultaneous Equation method was applied for the spectrophotometric determination of ACL and PNT. Statistical calculations were done by use of 276 nm and 289 nm wavelengths. In RP-HPLC method, the estimation of ACL and PNT carried out by ACN: Buffer (Phosphate acetate) (80:20 v/v) as mobile phase, pH 6.8 at a flow rate of 1.0 mL/min and YMC Triart C18 column (250 mm × 4.6 mm, 5 µ), column. The detection of ACL and PNT was carried out at 295 nm. The retention time of ACL and PNT was found at 3.739 and 2.991, respectively. The results of analysis in both the methods were validated as per ICH guidelines in terms of linearity, range, accuracy, precision, LOD, LOQ, ruggedness, robustness, and solution stability; from this study, it was concluded that the developed method of RP-HPLC can be successfully used for the estimation of ACL and PNT in their combined tablet formulations.
Devendra Singh *, Vivek Chauhan and Sapna Chaudhar
Department of Pharmacy, IIMT College of Pharmacy, Greater Noida, Uttar Pradesh, India.
06 April 2021
21 June 2021
15 June 2022
01 July 2022