DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF GLIPIZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORMSAbstract
A selective, simple, accurate and reproducible spectrophotometric method has been developed for the estimation of Glipizide in bulk and pharmaceutical formulation. Glipizide is a second generation sulfonylurea which lowers blood glucose in patients with diabetes mellitus type II. The drug obeyed the Beer’s law and showed good correlation. It showed absorption maxima at 276 nm in 0.1N NaOH. The developed method was validated with respect to linearity, accuracy and precision in accordance with the requirements of ICH guidelines. The linearity was observed between 10-30μg/ml having line equation Y=0.0283X – 0.0248 with correlation coefficient of 0.999.The limit of quantification and limit of detection were found to be 1.643 and 0.542ug/ml respectively. Moreover, the proposed analytical method is thus potentially useful for a routine laboratory because of its simplicity, rapidity, precision and accuracy.
Vaishali V. Karkhanis*, Anandkumari D. Captain and Priti H. Patel
A.R. College of Pharmacy and G.H. Patel Institute of Pharmacy Vallabh Vidhyanagar, Dist: Anand-388120, Gujarat, India
09 January, 2013
09 April, 2013
25 April, 2013
01 May, 2013