DEVELOPMENT AND VALIDATION OF UV-SPECTROSCOPIC METHOD FOR THE ESTIMATION OF LYMECYCLINE IN CAPSULE DOSAGE FORMAbstract
Lymecycline is a tetracycline broad-spectrum antibiotic. Present work describes a simple, accurate, precise, economical and reproducible spectrophotometric method in ultraviolet region has been developed and validated for the assay of Lymecycline in bulk and in Pharmaceutical formulations in diluent. Lymecycline is a tetracycline broad-spectrum antibiotic. Lymecycline like other tetracyclines is used to treat a range of infections Lymecycline exhibits absorption maxima at 270 nm in diluent. Beer’s law was found to be obeyed in the concentration range of 7.5-22.5 μg/ml. The optimum concentration of the Lymecycline was found to be 15 µg/ml. This concentration of Lymecycline was shown good absorbance valves at respective wavelengths was found to be 0.490. Linearity studies were carried out and the range was found to be 7.5-22.5 µg/ml for Lymecycline in diluent. The regression coefficient value of Lymecycline was found to be 0.999 which was not less than 0.995. The method is accurate, precise and economical. In this proposed method, there was no interference from common pharmaceutical excipients. The results of the analysis were validated statistically as per the ICH guidelines. The proposed method was successfully used for the routine analysis of the Lymecycline in bulk and in its capsule dosage form.
U. Narasimhulu *, M. Madhu, C. Sireesha, V. Vyshnavi and S. Rafi
Department of Pharmaceutical Analysis and Quality Assurance, Annamacharya College of Pharmacy, New Boyanapalli, Andhra Pradesh, India.
02 June 2019
05 September 2019
18 November 2019
01 April 2020