DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROMETRIC METHOD FOR ESTIMATION OF WATER SOLUBLE VITAMIN RIBOFLAVINAbstract
The present study describes a simple, accurate, precise and cost effective UV-Visible spectrophotometric method for the estimation of Riboflavin raw material. The Riboflavin is water soluble vitamin, so the solvent used throughout the experiment was 0.1N NaOH, the absorption maxima of drug was found at 445 nm. Beer’s law was obeyed in the range of 5ppm-30ppm. the developed method was successfully validated with respect to linearity, accuracy and precision. The method was validated and shown linearity in mentioned concentration. The correlation coefficient for Riboflavin was 0.999. The percentage relative standard deviation of inter-day precision range 0.66-1.04% and intra-day precision 1.05-1.39% both should be less than 2%. Hence proposed method was precise, accurate and cost effective, simple and rapid. This validated method can be applicable for quantitative determination of the titled drug with respect to assay from or for their solid dosage forms.
Himanshi Shah*, Shraddha Patel, Bhavik Patel, Nitin Solanki, Nurudin P. Jivani, Digbijay B. Kumar
Department of Quality Assurance, C.U. Shah College of Pharmacy and Research, Gujarat Technological University, Wadhwan-363 065, Gujarat, India
04 May, 2012
19 June, 2012
20 August, 2012
01 September, 2012