DEVELOPMENT, ESTIMATION AND VALIDATION OF GLIMEPIRIDE IN PHARMACEUTICAL FORMULATION BY HPLC METHOD
AbstractThe study is focused on developing a simple, rapid, validated High Performance Liquid Chromatographic (HPLC) method for Glimepiride Tablets from their dosage forms. The detection was carried out at 230 nm using Shimadzu UV – Visible detector HPLC system. The accuracy and precision were determined and validated statistically. The linearity was observed in the range of 15-120 µg/mL with a correlation coefficient of 0.999. The limit of detection and the limit of quantification were found to be 4 ng and 10 ng respectively. A Lichrosorb®(RP-18 column with a mobile phase consisting of acetonitrile – water – glacial acetic acid (550:450:0.6 v/v)) was used. The flow rate was 1 mL/min. The HPLC method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical industry quality control laboratories.
Article Information
14
3195-3198
400 KB
2139
English
IJPSR
Vania Maslarska
Medical University–Sofia, Faculty of Pharmacy, Department of Chemistry, Sofia, Bulgaria
vmaslarska@mail.bg
07 February, 2014
28 March, 2014
26 May, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(8).3195-98
01 August, 2014
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