DEVELOPMENT & EVALUATION OF SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF ATOVAQUONE IN PHARMACEUTICAL DOSAGE FORMAbstract
A very simple and selective U. V. Method was developed and validated for the estimation of Atovaquone in pure form and the nanosuspension. The adequate drug solubility and maximum assay sensitivity was found in 8.00 Ph IPA Phosphate Buffer in 40:60 ratios v/v. The absorbances were measured at the λmax. of 494 nm in the wavelength range of 200-800 nm. The linear calibration range was found to be 20-140 µg/ml. The same method was applied and validated for the determination of Atovaquone in nanosuspensions. Also no interference was found from the excipients added to the nanosuspensions at the selected wavelength of 494 nm and at the analysis condition .It was found that the developed method was accurate, sensitive, precise, robust and reproducible. It can be applied directly for the estimation of drug containing the pharmaceutical formulations.
Varsha H. Chungde*, Ajit A. Phalke, C. G. Kulkarni and K. B. Burade
Government College of Pharmacy Karad, India
27 May, 2013
09 July, 2013
26 September, 2013
01 October, 2013