DEVELOPMENT OF ANALYTICAL METHOD AND VALIDATION OF NADOLOLIN PURE AND PHARMACEUTICAL FORMULATIONS USING UV-SPECTROPHOTOMETRY AND SPECTROFLUORIMETRYAbstract
The development of analytical methods is in need for the estimation of drugs in pure and different pharmaceutical formulations. A simple, sensitive, rapid, accurate, precise and economic spectrophotometric and spectro-fluorimetric method was developed and validated for Nadolol in pure and pharmaceutical formulations. The proposed method was validated according to the International Conference on Harmonization (ICH) guidelines. The wave length (λmax) used for the estimation of Nadolol is 267 nm by spectrophotometry, excitation (λEx)-267 nm and emission (λEm)-300 nm by spectrofluorimetry. The linearity of the calibration curve was validated by the high values of the correlation coefficient of regression. The percentage of drugs recovered 100.37 ± 0.94% and 99.9 ± 0.59% for spectrophotometric and spectrofluorimetric methods respectively. LOD and LOQ values for Nadolol were found to be 3.531 µg/ml and 10.70 µg/ml by spectrophotometry and 0.45 µg/ml and 1.37 µg/ml by spectrofluorimetry. The developed methods are simple and suitable for the determination of Nadolol in pure and pharmaceutical preparations.
V. Veeramanikandan, R. Arun and A. Antonsmith *
Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil Nadu, India.
23 July 2019
22 November 2019
29 February 2020
01 June 2020