DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ABACAVIR AND LAMIVUDINE IN COMBINED TABLET DOSAGE FORM USING MULTICOMPONENT MODEAbstract
A simple, accurate, precise, economical analytical method has been developed for the simultaneous estimation of abacavir and lamivudine in pure bulk drug and in combined tablet dosage form by UV spectrophotometry in multicomponent mode. The standard solutions of abacavir and lamivudine in mixture of acetonitrile and methanol were diluted with distilled water individually to get the concentration of (20 µg/mL and 10 µg/mL) and the scanning range between 200 nm to 350 nm was selected. The sampling wavelengths 224, 241, 257, 280 and 296 nm was selected and the concentrations of individual drugs in five mixed standard solutions were fed to the multicomponent mode of the instrument. The results of analysis have been validated as per ICH guidelines and were found to be satisfactory. Hence, present study gives excellent methods for the determination of both the drugs in combined tablet formulation with relative ease.
V. P. Nagulwar* and K.P. Bhusari
Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur-441 110, Maharashtra, India
09 March, 2012
20 June, 2012
27 June, 2012
01 July 2012