DIETHYLSTILBESTROL (DES) INDUCED ENDOMETRIOSIS IN BALB/C MICE TREATED WITH MMP-2 AND 9 INHIBITORS-IAbstract
Diethylstilbestrol (DES) is an endocrine disturbing compound it can mimic and perform like a natural hormone, which mimics estradiol by binding to the estrogen receptor. This compound mainly reproductive target, so in this study used to induce endometriosis. Endometriosis is an estrone dependent constant gynaecological disorder. Here it, intends to induce endometriosis and treated by the MMP inhibitor to check whether it could attain the implantation or not. Hence the present study induces endometriosis by three different concentrations such as 10, 15 and 20 µg/kg body weight for 20 days at alternatively while to check the estrous cycle, hormonal regulation at 7th, 15th and 20th day also to record the body weight and reproductive organ weight from initial to final day of DES induction. Endometrial lesion developments were confirmed by anatomical observation. The above result reveals 20 µg DES induced group leads to endometrial cancer, so other 10 & 15 µg DES induced group carry out to further analysis. The endometrial lesions were treated by 10 µM concentrations of MMP 2 & 9 inhibitor I by interaperitonealy at 7 days. The determination of protein band and proteolytic activity of matrix metallo protein by SDS-Page and gelatin zymogram, to promote the histogram which is an intensity of protein band by using excel. Based on the results we consolidated 15 µg des induce group can induced the endometrial lesion and it treated by MMP 2 & 9 inhibitor compound to attain implantation to produce young ones. Further these reports were confirmed by vaginal protein, implantation attained animals and histopathological result.
M. Rajeswari and B. Kadalmani *
Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
27 September, 2017
26 April, 2018
13 May, 2018
01 July, 2018