DIRECT SHOOT REGENERATION FROM INTER NODAL EXPLANTS OF MENTHA VIRIDIS L.Abstract
Adventitious bud regeneration from internode explants of Mentha viridis L. was achieved shoots from nodal segments grown in vitro, were cut in to pieces and uses as sources of explants. Organogenesis was induced from internodal explants cultured on Murashige and Skoog (MS) medium containing different combinations of 6, Benzylaminopurine (BAP) and Kn under 16 hr photoperiod and at a temperature of 22 ±2 Co. The types of explants markedly influenced organogenesis and growth of the regenerated shoots. The regeneration frequencies were high in intermodal explants. The percentage of responsive in internodal explants producing from 20 to more than 91. BAP 3.5 mg/l induced the highest regeneration rate 91% and also get results of KN 3mg/l, regeneration rate 81 after 20d of culture. Yielding 36.7±1.14 shoots per responsive internode explants. The regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for 30d. Finally the elongated shoots were separated and transferred on to MS medium supplemented with (IBA, 1.5 mg/l). Rooted plantlets (90%) were successfully transferred to soil in cups with an 80% survival rate.
K. Senthil* and M. Kamaraj
P.G. and Research Department of Botany, Jamal Mohamed College (Autonomous), Tiruchirappalli- 20, Tamil Nadu, India
17 December, 2011
10 January, 2012
30 March, 2012
01 April, 2012