DISCOVERY OF INTERLEUKIN-6 INHIBITORY PROPERTIES OF THE COMPOUNDS DETECTED FROM METHANOLIC LEAF EXTRACTS OF PLECTRANTHUS AMBOINICUS (LOUR) SPRENG. THROUGH MOLECULAR DOCKING

Plectranthus amboinicus, locally called Karpooravalli in Tamil Nadu, India, is known for its various traditional therapeutic properties. Rheumatoid arthritis is a chronic autoimmune disease where uncontrolled inflammatory markers are released to induce bone damage and pain. In this study, the methanolic extract of P. amboinicus was characterized phytochemically and subjected to in-silico analysis to evaluate its anti-inflammatory properties. The methanolic extract of P. amboinicus was subjected to gas chromatography-mass spectrometry (GCMS) analysis. The detected compounds were subjected to in-silico analysis with biomarkers specific for rheumatoid arthritis (RA) that is interleukin 6. Of the 86 profiled compounds, three belonging to flavonoid, porphyrin, and heterocyclic triazoles classification had the highest affinities for interleukin-6 and was compared with common DMARs given during RA treatment which had significantly low affinities to above 3 highest affinities bioactive compounds. The ADMET profile, drug-likeness, and bioactivity score were also calculated for all the GCMS profiled compounds, revealing adequate drug-like properties. In-silico analysis of the leaf extract of P. amboinicus compounds showed a promising result for its anti-inflammatory activities and indicates the possible use of P. ambionicus extract in treatment of Rheumatoid arthritis.