DIURETIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF FRAGARIA ANANASSA FRUIT IN WISTAR ALBINO RATSAbstract
Fragaria ananassa fruit are small shrubs belonging to family rosaceae. Fragaria ananassa mainly contains anti-oxidants such as tocopherols, ascorbic acid and b-carotene which are effective against cancer, heart disease and cataracts. The focus of the study was toanalyze the diuretic activity of hydro alcoholic extract of Fragaria ananassa fruit in albino rats. Diuretic activity of hydroalcoholic (70:30) extract of Fragaria ananassa fruit (100mg/kg and 200mg/kg body weight orally) was studied in male wistar albino rats (n=5).Group I Serve as control receive 1% CMC solution, Group II serve as standard receive Furosemide 10mg/kg, Group III receive 100mg/kg Fragaria ananassa and Group IV receive 200mg/kg Urine volume at various interval was measured using metabolic cages (Lipschitz Method). The concentration of Na+, K+ in the urine at the end of 24 hours was estimated using flame photometer. One-way ANOVA technique was used for statically data analysis by which was followed by Dunnet t test (Graph Pad Prism software). On the basis of analysis, it was found that hydroalcoholic extract of Fragaria ananassa fruit showed a significant (P<0.05) dose dependent increase in urine volume. At 100 mg/kg and 200mg/kg hydroalcoholic extract of Fragaria ananassa fruit increased the excretion of sodium but decreased the excretion of potassium significantly compared to control.
P. Lodhi, A. Maru *, A. Joshi, S. Malviya and A. Kharia
Modern Institute of Pharmaceutical Sciences, Gram Alwasa, Indore, Madhya Pradesh, India.
18 August 2018
28 November 2018
17 January 2019
01 August 2019