DMSO INDUCED PRODUCT RECOVERY OF BACOSIDE A IN CELL SUSPENSION CULTURE OF BACOPA MONNIERI LINN.Abstract
Bacopa monnieri Linn. is an important medicinal plant belonging to family scrophulariaceae. Bacopa is a neurotonic, immuno-modulator, tranquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic. It is also used in Epilepsy, anxiety and depression. Major component of Bacopa are saponins Bacoside A and Bacoside B. The cell suspension culture of Bacopa monnieri was treated with 0.2%, 0.6% and 1% concentrations of DMSO (Dimethyl sulphoxide), a cell permeabilizing agent for 3 and 6 hours. The samples were analyzed for the content of bacoside A using HPTLC technique to identify and quantify the amount of released product. Maximum release of bioactive compound, Bacoside A was observed (4.6±0.02µg/mg) in suspension culture treated with 1% DMSO for 3 and 6 hrs treatment durations compared to control (2.1±0.05 and 3.2±0.01, respectively).
V. D. Mendhulkar* P. S. Patade and S. N. Singh
Department of Botany, Institute of Science, Mumbai, Maharashtra, India
01 July, 2011
10 August, 2011
26 October, 2011
01 November, 2011