DNA BARCODING, DETERMINATION OF BIOACTIVE COMPOUNDS, ANTIOXIDANT AND ANTI-DIABETIC PROPERTY IN EDIBLE GASTROPOD BROTIA COSTULA (RAFINESQUE, 1833) OF DIMAPUR DISTRICT, NAGALAND

The present study evaluated the bioactive components, antioxidant and anti-diabetic potential in the methanolic extract of edible Brotia costula. The identification of the sample was confirmed with the help of the DNA barcoding technique. The COI gene sequence was submitted in NCBI and obtained the accession number OM056887. Bioactive compounds present in the sample were evaluated and identified with the help of the Gas chromatography-mass spectroscopy (GC-MS) technique. The methanolic extract was tested for antioxidant capacity by its scavenging activity against Diphenylpicrylhydrazyl (DPPH), and its anti-diabetic activity was measured by the methanolic extract Alpha- amylase inhibition method. The GC-MS study revealed the presence of 76 bioactive components, and the compound with the highest peak area is phenol, 2, 4-bis (1, 1-dimethylethyl)-, phosphite. An inhibition percentage of 69.89 % in 500 μg against DPPH of the sample was observed as compared to 85.77% in ascorbic acid. The sample showed a good anti-diabetic potential with an inhibition percentage of 87.80 % in 500 μg of the sample. The findings of this study indicate that the methanolic extract of Brotia costula contains bioactive components and shows efficient antioxidant and anti-diabetic activity, which could be further utilized for various pharmaceutical purposes.