DUAL ROLE OF ARJUNOLIC ACID AND B12 IN MITIGATING SODIUM ARSENITE INDUCED MALFUNCTION OF FEMALE REPRODUCTIVE ORGANSAbstract
The present study was proposed to delineate the curative role of arjunolic acid and vitamin B12 alone or in combination against arsenic induced oxidative stress and female reproductive dysfunction. The Wistar strain adult female rats were treated with sodium arsenite at the dose of 1.0 mg/100 gm body weight orally for first eight days. Next eight days rats were treated with arjunolic acid (1.0 mg/100 gm body weight) and vitamin B12 (0.09 μg/100 gm body weight) orally. In ovarian and uterine tissue showed a recovery in arsenic induced ROS-production following the post-treatment of arjunolic acid and vitamin B12. Antioxidant status was also improved by arjunolic acid and vitamin B12 as established from the electrozymographic analysis of SOD, catalase and GPx. Post-treatment of these two significantly retrieved ovarian estradiol receptor 1 (ESR-1) from arsenic induced down regulation. Arjunolic acid and vitamin B12 also showed its curative ability in improving the level of inflammatory markers such as NF-κB, metallothioneine-1 (MT-1), TNF-α, IL-6. Thus these two bioactive substances might have the curative role against arsenic induced female reproductive dysfunction.
M. Maity, H. Perveen and S. Chattopadhyay *
Department of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics division (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal, India.
11 October 2018
11 January 2019
15 January 2019
01 July 2019